SERS substrates 

 Code Description Substrate Size, mm2 Thickness, mm Active area, mm2Price, EUR/pc**
(recommended Raman excitation wavelength 785 nm.)
 Au nanoparticles coated on ITO glass
(all substrate surface is conductive)
Conductivity </= 50 Ohms/sq.
 ITO glass25x25 1 25x25 100

(recommended Raman excitation wavelength 785 nm.)

 Au nanoparticles coated on microscope slide glass25x76 1 25x20* 50

* available custom size.

** 10% discount for each unit when the order quantity exceeds 20 pcs. 

  • A variety of substrate materials are obtainable.
  • Ag nanoparticles coated on a microscope slide are available.
  • Different sizes of nanoparticles are possible.

SERS substrates are prepared using laser-based technology. The typical Raman enhancement factor of SERS based on gold nanoparticles is 106-107.

Recommendations for the use of substrates: 

  • do not exceed 5 mW of laser average power using 50x(NA=0.5) objective during the measurements in Raman microscopy. 
  • use the substrates within 2 months from the date of the production.


  • Raman spectroscopy
  • Electrochemistry
  • Chemical identification
  • Environment measurements
  • Medical diagnostics


Fig. 1. a) SEM micrograph of ITO substrate coated with Au nanoparticles; b) and c) SEM images of a glass substrate coated with different size and density of Au nanoparticles. 


The comparison of Raman spectra of 2-Mercaptobenzoic acid on the ITO glass coated with Au nanoparticles (red curve) and on the ITO glass coated with a 10 nm gold film (blue curve) shown in Fig. 2a. 

Fig. 2. Raman spectra of 2-Mercaptobenzoic acid on ITO substrate No. AUITO-1 (a) and glass substrate No. AUG-2 (b).

SERS substrate enhances the Raman scattering light from molecules, capable of detecting trace amounts of chemicals and identifying them based on their unique vibrational characteristics.

Measurement conditions of Raman spectra:

Sample preparation: SERS substrates were immersed in an ethanol solution of 1mM Merkaptobenzoic acid for 16 hours. Afterward, substrates were rinsed in ethanol (96%) and dried at room temperature under slow nitrogen gas flow.

Raman measurement: laser wavelength 785 nm; average power 0.9 mW; 50x objective (NA=0.5); grating 1200 l/mm; integration time 100 s.